human mouse phospho stat1 tyr701  (Cell Signaling Technology Inc)

Journal: International Journal of Molecular Sciences

Article Title: Metabolic and Antioxidant Modulation by Artemisia indica Willd. Aqueous Extract in Glucose and Cholesterol Dysregulation

doi: 10.3390/ijms27010297

Figure Lengend Snippet: AAE decreased lipid accumulation by modulating SREBP-1 and FAS expression in HFD/STZ mice. ( a ) Total cholesterol (TC). ( b ) Total triglycerides (TGs). ( c ) High-density lipoprotein cholesterol (HDL-c). ( d ) Low-density lipoprotein cholesterol (LDL-c). ( e ) The ratio of TC/HDL-c. ( f ) The ratio of LDL-c/HDL-c. ( g ) The pathological changes examined through Oil Red O illustrate lipid accumulation (400× magnification). ( h ) Oil Red O-positive area. ( i ) Western blot analysis was performed to determine the protein levels of SREBP-1 and FAS, with β-actin used as a loading control. Data were collected per group and are presented as the mean ± SD ( n = 5), derived from five independent biological replicates. # p < 0.05, ## p < 0.01 versus the control group; * p < 0.05, ** p < 0.01 versus the model group. AAE: Artemisia indica Willd. aqueous extract; FAS: fatty acid synthase; HFD: high-fat diet; SREBP-1: sterol regulatory element-binding protein 1; STZ: streptozotocin.

Article Snippet: The membranes were incubated overnight at 4 °C with primary antibodies including p-PKCα, PKCα, NF-κB, SREBP-1, and FAS (all from Santa Cruz, CA, USA), as well as anti-β-actin (A5441) from Sigma-Aldrich.

Techniques: Expressing, Western Blot, Control, Derivative Assay, Binding Assay